The effect of tetrahydrofuran as solvent on matrix-assisted laser desorption/ionization and electrospray ionization mass spectra of functional polystyrenes.

Investigating Solvent-Induced Structural Transitions of Lysozyme in an Electrospray Ionization Source

Rapidly switchable matrix-assisted laser desorption/ionization and electrospray quadrupole-time-of-flight mass spectrometry for protein identification.

We describe a new interface for a prototype quadrupole-quadrupole-time-of-flight (TOF) mass spectrometer (Centaur, Sciex) that allows rapid switching between electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) modes of operation. Instrument performance in both modes is comparable (i.e., resolution approximately 10,000 FWHM, mass accuracy <10 ppm, sensitivity ap...

Supporting Information Selenocysteine in Native Chemical Ligation and Expressed Protein Ligation

Instruments. NMR spectra were obtained with Bruker AC-300 and Varian UNITY-500 spectrometers. Mass spectra were obtained with Perkin Elmer Sciex API 365 electrospray ionization (ESI) and Bruker Biflex III matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instruments at the University of Wisconsin Biotechnology Center. Ultraviolet and visible absorbance was recorded with a C...

Solvent-free mass spectrometry for hydrophobic peptide sequence analysis and protein conformation studies.

The breakthrough in soft ionization methods for biopolymers, electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) (1,2), revolutionized mass spectrometry (MS). As a result of these effective bioanalytical technologies, the entire field of proteomics changed, and the use of mass spectrometers for protein identification by accurate mass measurements or de novo seq...

Comparative mass spectrometric analyses of Photofrin oligomers by fast atom bombardment mass spectrometry, UV and IR matrix-assisted laser desorption/ionization mass spectrometry, electrospray ionization mass spectrometry and laser desorption/jet-cooling photoionization mass spectrometry.

Photofrin (porfimer sodium) is a porphyrin derivative used in the treatment of a variety of cancers by photodynamic therapy. This oligomer complex and a variety of porphyrin monomers, dimers and trimers were analyzed with five different mass spectral ionization techniques: fast atom bombardment, UV and IR matrix-assisted laser desorption/ionization, electrospray ionization, and laser desorption...